Fluorescence quenching at high quencher concentrations

Fluorescence Quenching at High Quencher Concentrations

Fluorescence quenching mechanisms in micelles: the effect of high quencher concentration

In this investigation the fluorescence quenching of fluorescent probe molecules situated within surfactant micelles was examined. The hydrophobic polyaromatic hydrocarbon benzo(a)pyrene (B(a)P) and the more polar 6-propionyl-2-(dimethylamino)-naphthalene (PRODAN) were used to assess the effect of high quencher concentration on the quenching process in micelles. Using the surfactant systems sodi...

Quinine Fluorescence Quenching at Low Ionic Strength

The effect of ionic activity on the quenching of the quinine dication fluorescence by chloride and bromide ions is examined in solutions of sufficiently low ionic strength to enable ionic activities to be taken into account by means of the Debye-Hückel equation. An equation for the dependence of the Stern-Volmer constant on the ionic strength is derived in a novel way and an iterative, linear l...

Fluorescence correlation spectroscopy at high concentrations using gold bowtie nanoantennas

Fluorescence correlation spectroscopy (FCS) measures the fluorescence fluctuations of fluorophores in solution, but is restricted to extremely low concentrations. Plasmonic gold bowtie nanoantennas enhance a single molecule’s fluorescence relative to a large background of unenhanced molecules, and here we show that bowties can extend FCS measurements to much higher concentrations. In this demon...

Quenching of tryptophan and 4,4′-diaminostilbene fluorescence by dinitrophenyl ethers: Use of 1-allyloxy-2,4-dinitrobenzene, as a quencher

The nitrophenol derivatives have multiple biological effects and their behaviour in environment is of interest. The fluorescence quenching property can be taken into consideration to understand dinitrophenyl ether toxicity. This study employs fluorescence spectroscopy to characterize the interaction of a model compound for dinitrophenyl ethers, namely 1-allyoxy-2,4-dinitrobenzene with two fluor...